Question 1Written preparation only: an eyepiece reticle is calibrated so that 25 divisions equal 100 μm on a stage micrometer. A cell spans 18 divisions with the same objective and optical configuration. Calculate its length, explain why the calibration does not automatically apply with another objective, and describe two precautions for preparing and observing a simple teacher-approved plant-tissue mount.5 indicative marks
Written preparation only: an eyepiece reticle is calibrated so that 25 divisions equal 100 μm on a stage micrometer. A cell spans 18 divisions with the same objective and optical configuration. Calculate its length, explain why the calibration does not automatically apply with another objective, and describe two precautions for preparing and observing a simple teacher-approved plant-tissue mount.
Cambridge 2027: Partial conceptual match
Microscopy preparation and calibrated measurement: selected explicit written support for internal practical inventory row 1. The source inventory is section-level, not an exhaustive leaf-outcome ledger; one exercise does not establish every outcome or practical bullet. Practical row IDs are internal identifiers, not official numbered syllabus codes. Paper 5 assesses planning and analysis in writing, but adequate preparation requires supervised laboratory experience; actual apparatus use, specimen handling and performance are not demonstrated here. Independent teacher approval is pending.
- 9700: Paper 3 / Manipulation, measurement and observation: Specimen handling and actual microscopy
AI-assisted placement. Independent teacher approval pending.
Show indicative guidance
Worked guidance
- Divide the known distance by the reticle division count, then multiply by the cell's division count. Do not multiply by objective magnification a second time.
- A reticle division has no fixed specimen-scale length at every magnification; calibration applies to a particular optical configuration.
- A teacher must observe preparation and instrument handling to assess practical performance. This task assesses written planning and calibration arithmetic only.
Self-marking points
View marking points
- Calibration is 100/25 = 4.0 μm per division, so 18 divisions represent 72 μm.
- Changing the objective changes image scale and requires the appropriate recalibration.
- Use a thin, suitably mounted specimen and avoid trapped air or overlapping tissue that obscures observation.
- Begin at low power and focus safely without driving the objective into the slide; follow the risk assessment for glass and any stain.
- A description does not demonstrate actual mount preparation, focusing or microscope control.
Examiner tip
Use the stated assumptions and distinguish observed evidence from interpretation. Original AI-reviewed written preparation; independent teacher approval pending. Practical technique requires direct supervised observation.